Publisher : University of the Philippines Visayas
Place of publication : Miagao,Iloilo,Philippines
Publication year : 2008
Thematic : Species
Language : English
Note
Dispersal is a primary determinant of connectivity, the extent to which populations in different parts of a species’ range are demographically linked by the exchange of individuals. However, estimating the scale and extent of connectivity in the marine environment remains a fundamental challenge. Emerging technologies, among them molecular genetic approaches, allow more precise, albeit indirect, estimates of dispersal patterns in space and time than ever before. Microsatellites are short, repetitive nucleotide sequences, widely used as molecular markers to estimate level of population genetic diversity, variability and connectivity. We describe methods for the identification of microsatellite markers for the mottled spinefoot, Siganus fuscescens, a valuable fishery resource widely distributed in shallow, seagrass-dominated reef flats throughout the Philippine archipelago. A genomic library enriched for repeat sequences was constructed using a selective hybridization approach adapted from Hamilton et al (1999) and Glenn and Schable (2005). From this enriched library, 37 sequences containing repeats were identified, of which 12 polymorphic loci have been characterized. These microsatellites are characterized based on an initial analysis of 100 S. fuscescens adult individuals collected from four locations along the northwest Luzon coast.
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Encoded by : Mae Belen Llanza
