Publisher : Biodiversity Journal
Place of publication :
Publication year : 2019
Thematic : Species
Language : English
Note
In this work a total of 1331 fish samples belonging to 15 species (Engraulis encrasicolus,
Alaccia aurita, Loligo vulgaris, Trigla lyra, Conger conger, Merluccius merluccius, Zeus
faber, Lophius piscatorius, Sardina pilchardus, Lepidopus caudatus, Scorpaena scrofa,
Scomber scombrus, Trachurus trachurus, Todarodes sagittatus, Trachinus draco) from Sicilian commercialized in Sicily (FAO 37, FAO 37.1.3, FAO 37.1.1, FAO 37.2.2, FAO 37.3,
FAO27,FAO 41, FAO87), were examined for the detection of Anisakidae larvae and molecular
identification. The fish samples were examined for the research of nematodes by visual inspection and digestion method according to the EC Regulation 2075/2005. Detected larvae
were subjected to morphological identification through the optical microscopy. Subsequently,
the DNA was extracted and the molecular identification of the larvae was conducted by
RFLP-PCR of the nuclear ITS region (ITS-1, ITS-2 and 5.8 S subunit). The polymerase chain
reactions targeting the cytochrome c oxidase subunit II (cox2) was performed and subjected
to sequence reaction. A number of 370 larvae have been identified at species level, of which
330 belong to the Anisakis pegreffii, 23 to the Anisakis simplex sensu strictu, 6 to the recombinant genotype Anisakis pegreffii/simplex s. s., 1 to Anisakis physeteris and 10 to Hysterothylacium fabri. The data obtained provide interesting reflections on the presence of parasites
belonging to the Anisakidae family in fishery products marketed in the Sicilian territory. These
findings are an excellent tool for assessing and preventing possible risks due to the consumption of these products.
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Keywords : Tropical rainforest
Encoded by : Pauline Carmel Joy Eje
